Supplementary Figure S4.

Characteristics of fibroblasts in CSCC and CAde. (a) Heatmap displayed the normalized enrichment scores (NES) of enriched terms in fibroblastic subclusters based on GSEA analysis. (b) Heatmap presented the expression levels of markers genes in each fibroblastic subcluster identified in fibroblast. (c) Bar plots displayed the proportions of each fibroblastic subcluster in individual samples. (d) Heatmap displayed the expression profiles of some functional gene set in fibroblastic subclusters. (e) Developmental trajectory of fibroblastic subclusters constructed by monocle 2, colored by subclusters. (f) Kaplan–Meier curves regarding the overall survival of myoCAFs based on their gene signature in CSCC cohort (left) and CAde cohort (right) from the TCGA cohort. The P-values were calculated by Log-rank test. (g) Kaplan–Meier curves regarding the overall survival of vCAFs based on their gene signature in CSCC cohort (left) and CAde cohort (right) from the TCGA database. The P-values were calculated by Log-rank test. (h) Flow cytometry plots of myoCAFs (left: POSTN⁺/COL1A1⁺) and vCAFs (right: SOD2⁺/COL1A1⁺) from one CC sample (Patient #46). (i) Flow cytometry plots of myoCAFs (left: POSTN⁺/COL1A1⁺) and vCAFs (right: SOD2⁺/COL1A1⁺) from another CC sample (Patient #47). (j) Transwell migration assay of CC cells treated with different condition medium from primary myoCAFs and vCAFs, respectively. Transwell assay had three independent biological replicates. ∗∗∗P < 0.001. The P-values were calculated by the Wilcoxon Rank Sum Test. (k) Blood vessel formation assay of HUVEC treated with different condition medium from primary myoCAFs, vCAFs or Con group. ∗P < 0.05, ∗∗P < 0.01. Blood vessel formation assay had three independent biological replicates. The P-values were calculated by the Wilcoxon Rank Sum Test.