Supplementary Figure S5.
Characteristics and subclusters of endothelial cells (ECs) in CSCC and CAde. (a-b) t-SNE plots of ECs, colored by cell types and individual samples, respectively. (c) Dot plots presented the normalized expression levels of marker genes in each EC subcluster. (d) Tissue preference of identified EC subcluster revealed by Ro/e (ratio of observed cell number to expected cell number). (e) The box plot presented the composition comparison for each subcluster of ECs in CSCC and CAde. The P-values were calculated by Wilcoxon Rank Sum Test. (f–g) Kaplan–Meier curves of overall survival (left) and disease-free survival (right) based on the gene signature of lymphatic endothelial cells (LECs). The P-value was calculated by Log-rank test. (h) IHC staining of LECs (PROX1) in collected CSCC (n=32) and CAde (n=11) samples. ∗ P < 0.05. The P-value was calculated by Wilcoxon Rank Sum Test. (i) Heatmap displayed the functional activities of identified EC subclusters based on known gene signatures. The upper panel depicted the average expression levels of representative genes in pathologic types, and the lower panel depicted the average expression levels of representative genes in each identified cell subcluster.