Fig. 1.

Construction of single-cell expression atlas for cervical squamous cell carcinoma (CSCC) and adenocarcinoma (CAde) tissues. (a) The study design and flowchart. (b) t-distributed stochastic neighbor embedding (tSNE) plots displayed the nine major cell types of cervical tissues based on snRNA-seq. (c) Dot plots displayed the normalized expression levels of canonical markers in identified cell types. (d) t-SNE plots of all identified single cells, colored by individual samples. (e) The proportions of cell types in each individual sample (left) and pathologic types (right). (f) Inferring of copy number variation (CNV) of epithelial cells. The upper panel exhibited large-scale CNVs of single cells inferred based on vascular endothelial cells (1000 cells). The lower panel exhibited large-scale CNVs of single cells inferred based on epithelial cells (∼20% epithelial cells). (g) Violin plots displayed the CNV score of each epithelial cell subcluster. (h) The cell cycle status of each identified cell type.