Fig. 2.

Characteristics and functional status of T cells and macrophages in CSCC and CAde. (a–b) t-SNE plots of the T cells, colored by cell type and individual samples, respectively. (c) Heatmap displayed the expression profiles of marker genes in each identified T cell subcluster. (d) Box plots displayed the composition comparison of each T cell subcluster in CSCC and CAde. The P-values were calculated by Wilcoxon Rank Sum Test. (e) Violin plots depicted the estimated scores of different functional statues in identified T cell subclusters. ∗P < 0.05, ∗∗∗P < 0.001. The P-values were calculated by Wilcoxon Rank Sum Test. (f) Box plots displayed the cytotoxicity (left) and the exhausted (right) score of CD8⁺ T cells in CSCC and CAde. ∗∗∗P < 0.001. The P-values were calculated by Wilcoxon Rank Sum Test. (g) Violin plot indicated the cytotoxicity scores of CSCC and CAde from the TCGA cohort. ∗P < 0.05. The P-value was calculated by Wilcoxon Rank Sum Test. (h) Kaplan–Meier curve of overall survival based on the gene signature of cytotoxicity in all CC cohort from the TCGA database. The P-value was calculated by Log-rank test. (i) t-SNE plots of the myeloid cells, colored by cell type. (j) The fraction of each macrophage subcluster in eight individual samples. (k) Dot plots depicted the enrichment GO terms of each identified macrophage subcluster. (l) Heatmap displayed the functional activity scores of each macrophage subcluster based on known gene signatures. (m) Violin plot depicted the phagocytosis scores of CSCC and CAde. ∗∗∗P < 0.001. The P-value was calculated by Wilcoxon Rank Sum Test. (n) mIF staining of macrophages with phagocytotic function in collected CSCC (n = 32) and CAde (n = 11) samples. (o) Box plots displayed the number of Macro-C2 (MRC1⁺/CD68⁺) cells/field in CSCC and CAde. ∗∗∗P < 0.001. The P-value was calculated by Wilcoxon Rank Sum Test.