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. 2023 Oct 17;67:102932. doi: 10.1016/j.redox.2023.102932

Fig. 5.

Fig. 5

A, Detection of lipoic acid and mitochondrial markers (DLST, PDH-E1β, OGDH and fumarase) in the DLST KO H838 cells expressing WT hDLST, DLST Cys37Glu or DLST Cys178Glu (n = 3/group). Actin and Coomassie staining were used to verify even loading of the gel. B-G, Densitometric quantification of the mitochondrial markers shown in A relative to cytosolic protein levels (actin). H, Detection of lipoic acid moieties associated with DLST and DLAT (dihydrolipoyllysine-residue acetyltransferase, a subunit of the pyruvate dehydrogenase complex) in the BS of WT and Ndufs4 KO mice (n = 7–8). In addition to the mitochondrial fractions (“mito”), a lane with a cytosolic fraction (“c”) was included as a negative control. I, Normalization of the signal intensity in H, relative to fumarase to account for mitochondrial protein levels. Normalization to Coomassie did not alter the lipoylation status (not shown). In panel A and H, molecular weight markers are shown on the left side.