Oligonucleotides used in this work.
Oligonucleotide | Sequence 5′-3′ | Description |
---|---|---|
pET28a(+)-PfThrRS-Fwd | TGGTGCCGCGCGGCAGCC̲A̲T̲A̲T̲G̲CCAACTATTACTCTACCC | Designed to clone entire PCS of PfThrRS as a NdeI-XhoI fragment into pET28a(+) using Gibson assembly |
pET28a(+)-PfThrRS-Rev | CAGTGGTGGTGGTGGTGGTGC̲T̲C̲G̲A̲G̲TTACTCCGAATCTGGGCG |