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. 2023 Aug 21;4(11):926–941. doi: 10.1039/d3cb00127j

Oligonucleotides used in this work.

Oligonucleotide Sequence 5′-3′ Description
pET28a(+)-PfThrRS-Fwd TGGTGCCGCGCGGCAGCC̲A̲T̲A̲T̲G̲CCAACTATTACTCTACCC Designed to clone entire PCS of PfThrRS as a NdeI-XhoI fragment into pET28a(+) using Gibson assembly
pET28a(+)-PfThrRS-Rev CAGTGGTGGTGGTGGTGGTGC̲T̲C̲G̲A̲G̲TTACTCCGAATCTGGGCG