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. Author manuscript; available in PMC: 2023 Nov 1.
Published in final edited form as: Am J Transplant. 2011 Nov 9;12(3):600–609. doi: 10.1111/j.1600-6143.2011.03845.x

Figure 3: ESC-derived T cells respond normally to stimulation.

Figure 3:

(A) In vitro generated T cells were immunophenotyped by flow cytometry (n = 6). The cells were shown to express CD45, CD3, αβ TcRs and γδ TcRs. Single CD8+, but no single CD4+ T cells were detectable. (B) To determine T-cell responses to stimuli, HoxB4-T cells and thymocytes were stimulated with antibodies against CD3 and CD28 or with PHA (n = 5). Both cell types responded well to mitogenic stimulation. (C) We determined whether the HoxB4-T cells express markers of activation. T cells were stimulated with either CD3/CD28 antibodies or with PHA and stained for CD69 after 48 hours. CD69 was upregulated in both cases confirming their responses to stimulation. These experiments were repeated at least 6 times.