Table 1. Crystallization conditions.
| Protein | Human DHX9 (150–1150) | Dog DHX9 (151–1151) | Cat DHX9 (151–1151) |
|---|---|---|---|
| Method | Hanging drop | Hanging drop | Hanging drop |
| Plate type | 24-well | 24-well | 24-well |
| Temperature (K) | 291 | 291 | 291 |
| Protein concentration (mg ml−1) | 8 | 8 | 8 |
| Buffer composition of protein solution | 20 mM HEPES, 300 mM NaCl, 2 mM TCEP pH 7.5, 5 mM ADP, 50 mM MgCl2 | 20 mM HEPES, 300 mM NaCl, 2 mM TCEP pH 7.5, 5 mM ADP, 50 mM MgCl2 | 20 mM HEPES, 300 mM NaCl, 2 mM TCEP pH 7.5, 5 mM ADP, 50 mM MgCl2 |
| Composition of reservoir solution | 200 mM lithium sulfate, 100 mM MES pH 6, 20%(w/v) PEG 4000 | 200 mM lithium sulfate, 100 mM MES pH 6, 20%(w/v) PEG 4000 | 150 mM ammonium sulfate, 100 mM Tris pH 8, 19%(w/v) PEG 4000 |
| Volumes of protein/reservoir in crystallization drop (µl) | 1.5/1.5 | 1.5/1.5 | 1.5/1.5 |
| Volume of reservoir (µl) | 500 | 500 | 500 |