Table 1. Data collection statistics and parameters used for spot finding.
Lysozyme | Lysozyme | Lysozyme | Lysozyme | KR2 | KR2 | |
---|---|---|---|---|---|---|
1 kHz | 0.1 kHz | 2 kHz subset | 2 kHz | Dark | Probe | |
Frame rate (Hz) | 1000 | 100 | 2000 | 2000 | 1000 | 1000 |
Collection time (s) | 100 | 1000 | 5 | 250 | 270 | 270 |
Photon energy (keV) | 15 | 15 | 11 | 11 | 11 | 11 |
Photon flux (photons s−1) | 6.0 × 1012 | 6.0 × 1011 | 1.2 × 1013 | 1.2 × 1013 | 1.2 × 1013 | 1.2 × 1013 |
Beam transmission (%) | 100 | 10 | 100 | 100 | 100 | 100 |
Jet speed (mm s−1) | 2.5 | 0.22 | 4.9 | 4.9 | 1.5 | 1.5 |
Number of frames | 100000 | 100000 | 10000 | 500000 | 269302 | 269302 |
Number of indexed crystals | 93717 | 71623 | 5412 | 265411 | 29341 | 28812 |
Resolution (Å) | 55.90–1.58 (1.64–1.58) | 55.90–1.60 (1.66–1.60) | 39.15–2.05 (2.12–2.05) | 39.15–1.70 (1.76–1.70) | 58.89–2.30 (2.38–2.30) | 58.89–2.30 (2.38–2.30) |
Completeness (%) | 100 | 100 | 99.9 | 100 | 100 | 100 |
CC1/2 | 99.6 (56.6) | 99.8 (47.4) | 91.5 (52.2) | 99.8 (54.3) | 97.0 (47.3) | 96.5 (42.1) |
SNR ratio | 9.3 (0.7) | 10.6 (1.1) | 5.4 (1.7) | 16.3 (0.04) | 5.6 (0.25) | 5.5 (0.2) |
Spot-finding parameters | ||||||
Threshold (–threshold =) | 10 | 10 | 10 | 10 | 16 | 16 |
SNR ratio (–min-snr =) | 3 | 4 | 3 | 3 | 6 | 6 |
Minimum pixel count (–min-pix-count =) | 1 | 1 | 1 | 1 | 1 | 1 |