Figure 4.

Comparison of on-chip smFISH protocol with traditional off-chip method. (A) Representative images of eggs stained off-chip (top) and on-chip (bottom) with the smFISH protocol. Blue indicates DAPI and identifies nuclei by staining chromosomes. Red puncta indicate individual par-1 molecules with zoom-in views in inserts. Scale bars are 15 μm. (B-C) Quantification of image quality between off-chip and on-chip experiments using SNR and SBR of the puncta for each condition. The red line indicates the minimum acceptable SNR for smFISH quantification (SNR = 7). Error bars represent SD, n = 20 embryos.