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. 2023 Oct 23;8(20):e163022. doi: 10.1172/jci.insight.163022

Figure 3. Metabolic competition between PDAC tumor cells and CAFs.

Figure 3

(A and B) Correlations between the number of CAFs and the expression of LDHA (A) or LDHB (B) in PDAC patients (n = 190). (C) Growth assay performed with human CAFs stimulated with lactate in complete medium (n = 3). (D) Representative fluorescence microscopy images of PANC-1 (top) or PK8 (bottom) cells cultured with human CAFs and fluorescently labeled glucose. Scale bars: 50 μm. (E) Quantification of glucose uptake by PDAC cells or CAFs counted under a fluorescence microscope (n = 3). (F) Quantification of glucose uptake by PDAC cells or CAFs by flow cytometry. (G) Western blotting analysis of MCT1 expression in CAFs cocultured with PANC-1 or PK8 cells. (H) Quantification of the intensity of MCT1 in Western blot analysis in G (n = 3). *P < 0.05; **P < 0.01. A Student’s t test was used to compare continuous variables between 2 groups. One-way ANOVA followed by Tukey’s multiple-comparison test was used to compare multiple groups. 2NBDG, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose.