Figure 1.

Generation of osimertinib-resistant H1975 and HCC827 cells, H1975-OR and HCC827-OR. (A) Schematic outline of osimertinib-resistant cell line establishment, edited by Figdraw (https://www.figdraw.com/). The drug sensitivity of (B) HCC827 and HCC827-OR cells, and (C) H1975 and H1975-OR cells was detected by CCK8 assay. IC₅₀ values of osimertinib in (D) HCC827 and HCC827-OR cells, and (E) H1975 and H1975-OR cells were examined by CCK8 assay. The morphological differences between (F) HCC827 and HCC827-OR cells, and (G) H1975 and H1975-OR cells were observed under a light microscope. ^**P<0.01. CCK8, Cell Counting Kit 8; IC₅₀, half-maximal inhibitory concentration.