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. 2023 Sep 7;52(5):102. doi: 10.3892/ijmm.2023.5305

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Profiles of differentially expressed circRNAs in HCC827, HCC827-OR, H1975 and H1975-OR cells. Unsupervised hierarchical clustering analysis of the differentially expressed circRNAs between (A) HCC827 and HCC827-OR cells, and (B) H1975 and H1975-OR cells. Kyoto Encyclopedia of Genes and Genomes analysis for the host genes of differentially expressed circRNAs in (C) HCC827 and HCC827-OR cells, and (D) H1975 and H1975-OR cells. Rich factor represents the enrichment degree of differentially expressed genes. The y-axis shows the name of the enriched pathways. The area of each node represents the number of enriched host genes of differentially expressed circRNAs. The P-value is represented by a color scale. (E) HCC827 and HCC827-OR cells, and (F) H1975 and H1975-OR cells were treated with 10 µM RAPA or 1 nM 3-MA for 24 h at 37°C, and the expression levels of autophagy-related proteins were detected by western blot analysis. Western blots were semi-quantified. ^*P<0.05 and ^**P<0.01 vs. ctrl. 3-MA, 3-methyladenine; circRNA, circular RNA; ctrl, control; RAPA, rapamycin.