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. 2023 Sep 7;52(5):102. doi: 10.3892/ijmm.2023.5305

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Downregulation of circPPP4R1 increase the sensitivity of H1975-OR cells to osimertinib. The presence of circPPP4R1 was (A) validated by RT-qPCR in H1975 and H1975-OR cells, followed by (B) Sanger sequencing. (C) RT-qPCR analysis of circPPP4R1 expression levels in H1975-OR cells transfected with two discrete chemically synthesized siRNAs. H1975-OR and circPPP4R1-depleted H1975-OR cells treated with or without osimertinib (1 µM) were assessed by (D) Cell Counting Kit 8 assay, (E) colony formation assay, (F) Transwell assay and (G) tube-formation assays. ^*P<0.05 and ^**P<0.01 vs. H1975 or as indicated. NC, negative control; ns, not significant; RT-qPCR, reverse transcription-quantitative PCR; si, small interfering.