Figure 4.

Identification of a HA/HC complex within the mouse, human, and porcine limbus and cornea by Western blotting. Western blot analysis of mouse (A), human (B), and porcine (C) central cornea and limbal crude extracts were digested with hyaluronidase or not and subjected to SDS-PAGE, transferred to polyvinylidene difluoride membranes and stained with anti-IαI (DAKO). Undigested samples show a large HA complex at the origin, whereas in digested samples, bands carrying IαI heavy chain fragments are able to migrate into the gel.