TABLE 2.

Consistency and precision of the Ile assay

Sample	Amt (pmol) of amino acids as determined by AAAAa				Proportions of Ile compared with other amino acids
	Ile	Leu	Val	Ala	Ile/Leub	Ile/Valc	Ile/Alad	Avge
94.1A	0.775	441.72	366.64	446.37	0.126	0.125	0.125	0.125
94-1B	0.969	433.16	357.89	434.71	0.161	0.160	0.161	0.160
94-1C	0.527	431.99	358.57	435.29	0.088	0.087	0.087	0.087
94-2A	0.407	426.67	357.40	436.13	0.069	0.067	0.067	0.068
94-3A	0.633	452.58	374.31	446.11	0.101	0.100	0.102	0.101
94-3B	0.400	411.50	344.01	418.50	0.070	0.069	0.069	0.069

^aEach rHb1.1 molecule contains 72 leucine residues, 59 valine residues, and 72 alanine residues. 

^bCalculated as follows: (picomoles of Ile/picomoles of Leu) × 72. 

^cCalculated as follows: (picomoles of Ile/picomoles of Val) × 59. 

^dCalculated as follows: (picomoles of Ile/picomoles of Ala) × 72. 

^eThe overall average ± standard deviation was 0.102 ± 0.036, and the RSD was 35%.