TABLE 5.

Amino acid compositions of rHb1.1 and typical E. coli proteins

Amino acid	No. of residues in rHb1.1	μmol/g of rHb1.1	μmol/g of E. coli protein (avg)a	% Change in distributionb
Ala	72	1,159	887	8
Arg	12	193	511	−16
Asn	18c	290	416	−8
Asp	30	483	416	4
Cys	6	97	158	−10
Glu	24	386	455	−4
Gln	8	129	455	−18
Gly	41	660	1,058	−9
His	38	612	164	68
Ile	0	0	502	−25
Leu	72c	1,159	778	12
Lys	46c	741	593	6
Met	9c	145	265	−11
Phe	30c	483	320	13
Pro	28	451	382	5
Ser	32	515	373	10
Thr	32c	515	438	4
Trp	6	97	98	−0
Tyr	12	193	238	−5
Val	59c	950	731	7
Total	575	9,258	9,238	0

^aCalculated by using the data of Neidhardt and Umbarger (14). 

^bPercent change in amino acid distribution in the total protein of E. coli cells when the level of rHb1.1 expression was 25%. 

^cSome but not necessarily all of the codons for this amino acid are identical to an Ile codon at two of the three positions; 200 of the 575 codons tested were identical to an Ile codon at two of the three positions.