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. 2023 Sep;33(9):1482–1496. doi: 10.1101/gr.278073.123

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© 2023 Shi et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 2. — Tissue-level phenotypes of Zswim8^−/− embryos. (A) Heart defect in Zswim8^−/− embryos. Shown are representative images of H&E-stained transverse sections of hearts from Zswim8^+/− and Zswim8^−/− littermates at E18.5. Arrowhead points to a VSD. (B) Sacculation defect in Zswim8^−/− embryos. Shown are lung sections from Zswim8^+/+, Zswim8^+/−, and Zswim8^−/− embryos at the indicated developmental times immunostained for Podoplanin and Pro-surfactant C (green and magenta, respectively) and stained for DNA (Hoechst; blue). Scalebar represents 100 μm.