Fig. 3. Connectomic analysis of the lobula plate motion vision network.
a, Schematic of the Drosophila hemibrain volume (left). A part of the lobula plate is included in the reconstructed EM volume (middle, indicated in light blue). LPi3-4 cells are mainly presynaptic in layer 4 and postsynaptic in layer 3 of the lobula plate, while the opposite is true for LPi4-3 cells (right). b,c, Reconstructed LPi3-4 cells (partly fragmented) from the hemibrain dataset (b). Presynaptic (red) and postsynaptic (green) sites of LPi3-4 cells in relation to all synapses (blue) were detected in the corresponding EM volume (indicated with dashes in c). Relative positions of the lobula plate layers are indicated with numbers 1–4. d,e, Same as b,c but for LPi4-3 cells. f, Example of single LPi3-4, LPi4-3 and VS cells and their synaptic connections. g, Single EM section with cell profiles from cells in f highlighted in color. Scale bar, 2 µm. h, Connectivity matrix of the analyzed cell types. i, Percentage of synapses from T4/T5 and LPi cells onto other LPi or VS cells. j, VS cell conductance change to PD or ND visual stimulation (Extended Data Fig. 3). NS, not significant. k, Updated schematic of the lobula plate network. Newly identified synaptic connections are highlighted in red. Data in j: n = 16 VS cells. The dots indicate individual cells. The bars and error bars indicate the mean ± s.e.m. The statistical test used was a two-sided Wilcoxon signed-rank test.