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. 2023 Aug 7;17(11):2295–2313. doi: 10.1002/1878-0261.13493

Fig. 2.

Fig. 2

High‐throughput screen with MYC‐EBOX‐CRISPR and Brunello libraries. (A) Experimental approach: (1) MYC‐EBOX‐CRISPR library to destroy E‐box sequences, disrupt MYC binding, and its effect on target‐gene expression (2) Brunello library for genome‐wide gene knockout. (B) Scheme of the high‐throughput screen in cancer cells with MYC‐EBOX‐CRISPR and Brunello libraries. (C) DESeq2 analysis revealed essential genes in Brunello library and (D) essential E‐boxes in MYC‐EBOX‐CRISPR (depleted genes and E‐boxes in blue, enriched genes and E‐boxes in red). (E) Circos plots showing log2 fold change (FC) values for genes in Brunello screen (outer circle) and E‐boxes in MYC‐EBOX‐CRISPR screen (inner circle) across the chromosomes. Blue dots indicate genes and E‐boxes significantly (P adj < 0.001) depleted or enriched, black lines denote log2FC = 0. (A and B) were created using BioRender.