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. 2023 Nov 1;14:6983. doi: 10.1038/s41467-023-42672-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Schematic representation of mRNA delivery in vivo to assess cell specificity. Created with BioRender.com. Briefly, 15 µg of Cre mRNA were complexed with each CART and delivered retro-orbitally. Flow cytometric analysis of isolated spleens was performed 48 h after transfection. b Percentage of Cre-mediated recombination in CD45+ subsets in vivo (n = 3, bars represent mean values +/− SD). c Percentage of Cre-mediated recombination in CD4+ and CD8 + T cells in vivo (n = 3, bars represent mean values +/− SD). Statistical significance was calculated using one-way ANOVA with Tukey’s multiple comparisons test. Cell icons were created with BioRender.com. d Schematic representation of mRNA delivery in vivo to assess T-cell-specific luciferase expression. Created with BioRender.com. e Quantitation of luminescence signal in untreated (Unt.) splenic T cells or 6 h after transfection of 5 µg mRNA luciferase with ONA or bAC-7c (n = 3, bars represent mean values +/− SD). Statistical significance was calculated using one-way ANOVA with Dunnett’s multiple comparisons test. f Effect of mRNA dose in isolated splenic T cells 6 h after transfection with bAC-7c (n = 3, bars represent mean values +/− SD).