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. 2023 Nov 1;14:6949. doi: 10.1038/s41467-023-42582-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Biological process GO terms enriched for DEGs identified from the comparisons between symbiotic anemones incubated with or without 10 mM glucose (a) and aposymbiotic anemones incubated with or without 10 mM glucose (b). GO terms were clustered based on their semantic similarity. Word cloud summarizes the features with keywords in each GO cluster, with different font sizes representing the level of the enrichment. c Overrepresented pathways enriched in glucose-regulated DEGs in aposymbiotic and symbiotic E. diaphana. The enrichment score (ES) was calculated by dividing the actual by the expected number of DEGs associated with the corresponding pathway. The p-values were calculated with overrepresentation analysis implemented in the R package clusterProfiler. d Expression changes of hub genes involved in amino acid biosynthesis in response to symbiosis and glucose supplementation.