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. 2023 Nov 1;14:6977. doi: 10.1038/s41467-023-42464-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Microsynteny analysis of triticein BGC in wheat group 5 chromosomes, and syntenic regions in Aegilops tauschii and wild emmer wheat (T. turgidum). b The triticein BGC is conserved in Aegilops tauschii and four Aegilops grasses from section Sitopsis, but absent in A and B lineage genomes, including wild emmer wheat (T. turgidum ssp. dicoccoides), durum wheat (T. turgidum ssp. durum), and putative B genome progenitor, Aegilops speltoides. Color coding shows % identity of nucleotide sequence of top blastn alignment with the coding sequences of the respective BGC4(5D) genes. The phylogenetic tree is adapted from Li et al.³⁶. c Extracted ion chromatogram (EIC) and MS2 fragmentation for triticein (m/z = 329.1012) in bread wheat, emmer wheat and Aegilops tauschii. Digitoxin (m/z = 375.2530) was used as an internal standard. Y-axes of all chromatograms are linked. Cultivars/accessions analyzed, in order shown in figure from top to bottom: T. aestivum Cadenza; T. turgidum TRI 18539, PI 428127, PI 466991, CGN 16073, CGN21064, TRI 6141, Miradoux; A. tauschii subsp. strangulata TOWWC0190.