Skip to main content
. Author manuscript; available in PMC: 2023 Nov 2.
Published in final edited form as: J Cardiovasc Transl Res. 2021 Jul 20;15(1):167–178. doi: 10.1007/s12265-021-10153-5

Fig. 1. Histological staining reveals similar pathological remodeling in both humans and swine.

Fig. 1

Coronary artery histological sections from a human with non-ischemic <50% plaque burden (a-c), a human with an ischemic >75% plaque burden (d-f), a lean swine (g-i), and a MetS swine (j-l). Verhoeff-Van Gieson staining was used to determine plaque burden and the areas of the tunica media and intima using the visible external elastic lamina (EEL, red dashed line) and internal elastic lamina (IEL, yellow dashed line) (a, d, g, j). Von Kossa staining was used to determine percent of vascular calcification (black) (b, e, h, k). Trichrome staining was used to determine the percent collagen (blue) (c, f, i, l). Humans (bars and blue symbols) were binned based on percent plaque burden and swine (bars and red symbols) showed significantly increased plaque burden in the MetS group compared to the lean group (m). Percent vascular calcification was not significantly different between human or swine groups (n). The percent collagen increased with disease in both the human and swine groups (o). EEL, external elastic lamina; IEL, internal elastic lamina; M, tunica media; I, tunica intima; L, lumen.