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. 1998 May;64(5):1620–1627. doi: 10.1128/aem.64.5.1620-1627.1998

TABLE 3.

Catechol pathway enzyme activities of cell extracts of two strains growing on 1.5 mM 3CBAa

Genotype Enzyme Substrate Activity (μmol/mg of protein · min−1) % Activity
WV-4b Catechol dioxygenase Catechol 300 100
3-Chlorocatechol 49 16
4-Chlorocatechol 192 64
3,5-Dichlorocatechol 191 78
3-Methylcatechol 220 232
4-Methylcatechol 697 100
Muconate cycloisomerase Muconate 181 100
2-Chloromuconate 11 6
3-Chloromuconate 37 21
PM-1c Catechol dioxygenase Catechol 352 100
3-Chlorocatechol 5 2
4-Chlorocatechol 91 26
3,5-Dichlorocatechol 2 0.6
3-Methylcatechol 104 30
4-Methylcatechol 245 70
Muconate cycloisomerase Muconate 142 100
2-Chloromuconate 2 17
3-Chloromuconate 51 352
a

See text for details. 

b

Strain WV-7-301. 

c

Strain PM-P-301.