TABLE 3.
Genotype | Enzyme | Substrate | Activity (μmol/mg of protein · min−1) | % Activity |
---|---|---|---|---|
WV-4b | Catechol dioxygenase | Catechol | 300 | 100 |
3-Chlorocatechol | 49 | 16 | ||
4-Chlorocatechol | 192 | 64 | ||
3,5-Dichlorocatechol | 191 | 78 | ||
3-Methylcatechol | 220 | 232 | ||
4-Methylcatechol | 697 | 100 | ||
Muconate cycloisomerase | Muconate | 181 | 100 | |
2-Chloromuconate | 11 | 6 | ||
3-Chloromuconate | 37 | 21 | ||
PM-1c | Catechol dioxygenase | Catechol | 352 | 100 |
3-Chlorocatechol | 5 | 2 | ||
4-Chlorocatechol | 91 | 26 | ||
3,5-Dichlorocatechol | 2 | 0.6 | ||
3-Methylcatechol | 104 | 30 | ||
4-Methylcatechol | 245 | 70 | ||
Muconate cycloisomerase | Muconate | 142 | 100 | |
2-Chloromuconate | 2 | 17 | ||
3-Chloromuconate | 51 | 352 |
See text for details.
Strain WV-7-301.
Strain PM-P-301.