Table 1.
Ratio of cFos-TRAPed cells in subdivided brain regions and results of two-way ANOVA.
| Brain region | Acronym | Ratio (# of cells in each class 6 region/# of cells in each class 1–5 region) | p-value by two-way ANOVA | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Ad lib sleep | Sleep deprivation | Condition | Method | Condition × Method interaction | |||||
| Chamber | Hand | Chamber | Hand | ||||||
| Olfactory areas | Other olfactory areas | Other OLF | 3.820% | 4.531% | 3.680% | 4.121% | 0.0550 | 0.0049 | 0.2572 |
| Cortical subplate | Claustrum | CLA | 2.883% | 7.357% | 4.020% | 4.724% | 0.3987 | 0.0309 | 0.0761 |
| Basolateral amygdalar nucleus anterior part | BLAa | 16.296% | 15.120% | 15.423% | 11.745% | 0.0385 | 0.0256 | 0.1481 | |
| Striatum | Caudoputamen | CP | 27.470% | 28.719% | 31.707% | 42.050% | 0.0007 | 0.0033 | 0.0079 |
| Nucleus accumbens | ACB | 8.389% | 9.777% | 5.885% | 7.394% | 0.0012 | 0.0080 | 0.8488 | |
| Septohippocampal nucleus | SH | 0.064% | 0.063% | 0.000% | 0.013% | 0.0081 | 0.6494 | 0.5938 | |
| Other striatum | Other STR | 7.498% | 8.414% | 10.562% | 7.317% | 0.1565 | 0.1081 | 0.0211 | |
| Pallidum | Globus pallidus external segment | GPe | 9.399% | 8.969% | 11.484% | 7.482% | 0.6855 | 0.0320 | 0.0599 |
| Magnocellular nucleus | MA | 2.856% | 1.520% | 3.176% | 2.400% | 0.0988 | 0.0197 | 0.3744 | |
| Thalamus | Reticular nucleus of the thalamus | RT | 2.693% | 1.693% | 1.457% | 1.219% | 0.0298 | 0.0747 | 0.2142 |
| Epithalamus | EPI | 1.576% | 0.941% | 1.051% | 3.171% | 0.0204 | 0.0315 | 0.0038 | |
| Hypothalamus | Anteroventral preoptic nucleus | AVP | 0.195% | 0.273% | 0.781% | 0.152% | 0.0918 | 0.0592 | 0.0283 |
| Medial preoptic area | MPO | 3.051% | 3.803% | 2.739% | 4.916% | 0.4270 | 0.0321 | 0.1916 | |
| Vascular organ of the lamina terminalis | OV | 0.016% | 0.000% | 0.100% | 0.000% | 0.0126 | 0.0041 | 0.0126 | |
| Periventricular hypothalamic nucleus preoptic part | PVpo | 1.421% | 1.791% | 0.630% | 0.879% | 0.0069 | 0.1355 | 0.7346 | |
| Ventromedial preoptic nucleus | VMPO | 0.687% | 0.579% | 0.037% | 0.382% | 0.0073 | 0.2315 | 0.0544 | |
| Anterior hypothalamic nucleus | AHN | 4.714% | 5.904% | 7.658% | 4.289% | 0.1290 | 0.0353 | 0.0028 | |
| Lateral hypothalamic area | LHA | 9.209% | 9.078% | 10.692% | 10.790% | 0.0218 | 0.9711 | 0.8070 | |
| Preparasubthalamic nucleus | PST | 0.460% | 0.000% | 0.151% | 0.000% | 0.0910 | 0.0118 | 0.0910 | |
| Tuberal nucleus | TU | 3.781% | 3.291% | 3.749% | 2.130% | 0.1289 | 0.0279 | 0.1453 | |
| Midbrain | Superior colliculus optic layer | SCop | 5.812% | 4.009% | 3.863% | 5.086% | 0.4099 | 0.5732 | 0.0332 |
| Inferior colliculus dorsal nucleus | ICd | 2.570% | 4.477% | 2.256% | 5.311% | 0.6170 | 0.0067 | 0.2978 | |
| Parabigeminal nucleus | PBG | 0.000% | 0.000% | 0.044% | 0.012% | 0.0272 | 0.1277 | 0.1277 | |
| Ventral tegmental area | VTA | 0.371% | 0.434% | 0.749% | 0.772% | 0.0461 | 0.7514 | 0.8823 | |
| Superior colliculus motor related intermediate white layer | SCiw | 8.014% | 5.930% | 8.213% | 5.865% | 0.9097 | 0.0163 | 0.8245 | |
| Superior colliculus motor related intermediate gray layer | SCig | 10.550% | 7.829% | 8.515% | 7.814% | 0.1142 | 0.0282 | 0.1181 | |
| Medial terminal nucleus of the accessory optic tract | MT | 0.039% | 0.097% | 0.157% | 0.000% | 0.6909 | 0.1292 | 0.0144 | |
| Interpeduncular nucleus | IPN | 0.424% | 0.609% | 0.249% | 1.187% | 0.1336 | 0.0064 | 0.0247 | |
| Rostral linear nucleus raphe | RL | 0.312% | 0.182% | 0.156% | 0.101% | 0.0297 | 0.0628 | 0.3605 | |
| Pons | Pontine gray | PG | 35.926% | 27.559% | 23.416% | 35.250% | 0.5418 | 0.6569 | 0.0492 |
| Pontine reticular nucleus | PRNr | 4.051% | 3.631% | 6.604% | 2.657% | 0.3591 | 0.0458 | 0.0819 | |
| Other pons | Other pons | 16.009% | 24.533% | 17.390% | 25.570% | 0.5952 | 0.0164 | 0.9386 | |
| Medulla | Facial motor nucleus | VII | 6.44% | 6.08% | 4.48% | 1.40% | 0.0410 | 0.1988 | 0.2892 |
| Magnocellular reticular nucleus | MARN | 1.61% | 2.10% | 2.27% | 4.96% | 0.0255 | 0.0349 | 0.0962 | |
| Vestibular nuclei | VNC | 12.17% | 8.52% | 5.88% | 7.90% | 0.0358 | 0.5045 | 0.0634 | |
| Nucleus raphe magnus | RM | 1.19% | 0.74% | 1.59% | 0.10% | 0.5941 | 0.0091 | 0.0645 | |
| Cerebellum | Lobule III | CENT3 | 15.618% | 9.265% | 20.029% | 8.415% | 0.5045 | 0.0209 | 0.3401 |
| fiber tracts | oculomotor nerve | IIIn | 0.174% | 0.034% | 0.000% | 0.000% | 0.0164 | 0.0548 | 0.0548 |
| brachium of the inferior colliculus | bic | 0.528% | 0.911% | 1.898% | 0.643% | 0.1191 | 0.1930 | 0.0424 | |
| medial lemniscus | ml | 10.581% | 5.553% | 5.084% | 5.453% | 0.0242 | 0.0424 | 0.0271 | |
| genu of corpus callosum | ccg | 1.023% | 0.525% | 0.202% | 0.364% | 0.0252 | 0.2982 | 0.0792 | |
| corticospinal tract | cst | 0.000% | 1.652% | 0.000% | 0.925% | 0.4308 | 0.0361 | 0.4308 | |
| internal capsule | int | 4.575% | 2.929% | 4.141% | 2.850% | 0.6170 | 0.0364 | 0.7275 | |
| rubrospinal tract | rust | 1.913% | 1.037% | 1.299% | 0.943% | 0.0407 | 0.0066 | 0.0942 | |
| columns of the fornix | fx | 2.469% | 1.217% | 1.190% | 0.663% | 0.0000 | 0.0000 | 0.0000 | |
| mammillary peduncle | mp | 0.053% | 0.211% | 0.425% | 0.065% | 0.1547 | 0.1906 | 0.0158 | |
| ventricular systems | lateral recess | V4r | 7.836% | 9.559% | 1.793% | 15.837% | 0.9674 | 0.0436 | 0.0852 |
The ratios of cFos-TRAPed cells are shown for regions with p < 0.05 by two-way ANOVA. All data are available in Supplementary Table S3. Bold values denote statistical significance at the p<0.05 level.