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. 2023 Jun 1;108(11):3086–3094. doi: 10.3324/haematol.2023.282684

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Figure 1. — Presence of mitochondria in mature sickle red blood cells. (A) ImageStream and staining with Mitotracker Red CMRXRos dye of mitochondria in mature sickle red blood cells (RBC) of three sickle cell (SCA) patients. (B) ImageStream and staining with Mitotracker Red CMRXRos dye show no mitochondria in RBC from healthy donors. (C) Gating strategy for flow cytometry analysis to discriminate mature sickle RBC retaining mitochondria (CD71^- Mitotracker⁺). (D) Mean percentages of mature RBC with mitochondria (mito) in 61 SCA (SS) patients and 10 healthy (AA) donors. Significant difference: ****P<0.0001.