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. 2023 Jun 1;108(11):3086–3094. doi: 10.3324/haematol.2023.282684

Figure 2.

Figure 2.

Mitochondrial respiration in mature red blood cells from sickle cell disease patients. (A) Comparison of mitochondrial respiration between 5 healthy and 8 mature sickle red blood cells (RBC). (B) Effect of Antimycin A on superoxide anion generated by mitochondria in mature sickle RBC (N=13). Endogenous: basal mitochondrial O2 consumption; ATP-inhibited: mitochondrial O2 consumption after the use of ATP-synthase inhibitor (Oligomycin); uncoupled: maximal uncoupled O2 consumption obtained with the mitochondrial uncoupler (carbonyl cyanide-p-trifluoro-methoxyphenyl-hydrazone [FCCP]); non-mitochondrial: O2 consumption after adding the Antimycin A. Difference between healthy and mature sickle RBC: $P>0.05; $$$P<0.001. The addition of mitochondrial inhibitors or uncoupler had no effects on healthy RBC while changes were observed on mature sickle RBC: ***P<0.001. Difference between Mitosox and Mitosox+Antimycin A conditions: ***P<0.001. %RBC Mitosox+: percentages of RBC accumulating superoxide anion.