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. 2023 Jun 22;108(11):3011–3024. doi: 10.3324/haematol.2022.282474

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Figure 3. — Specific T-cell-EBI3 depletion promotes leukemia development. (A) Control (Ctrl) and Ebi3^-/- mice were euthanized, and CD3⁺ T cells were isolated from the splenocytes. Recipient Rag2^-/- mice were injected with either Ctrl (N=10) or Ebi3^-/- CD3⁺ T cells (N=10) and subsequently injected with splenocytes derived from leukemic Eμ-TCL1 mice. Experimental mice were bled weekly to evaluate peripheral disease development. Number of circulating neoplastic CD5⁺ CD19⁺ cells is shown in the 2 groups (two-way ANOVA test). (B) Percentage of chronic lymphocytic leukemia (CLL) cells in the spleen of recipient Rag2^-/- mice. (C) Percentages of CD8⁺, conventional T cells (Tconv), and regulatory T cells (Tregs) among the injected Ctrl and Ebi3^-/- CD3⁺ T cells. (D) Percentages of CD8⁺ T cells in clusters identified in spleens of recipient Rag2^-/- mice. (E, F) Frequency of Tregs and of CD25⁺ and KLRG1⁺ Tregs in the spleen of recipient Rag2^-/- mice. Unpaired t test, *P<0.05, ****P<0.0001.