Figure 1.

Molecular analysis of LSK cells uncovers cell cycle status heterogeneity in MPPs. (A) UMAP projection for CITE-seq–identified clusters and marker genes from the software ICGS2. Coloring is derived from the clusters identified in Fig. S1 B. (B) Gene set enrichment heatmap for the different ICGS2 cluster (GO-Elite), displaying z-scores relative to a prior curated set of HSPC subset genes (¹Rodriguez-Fraticelli et al., 2020; ²Giladi et al., 2018; ³Cabezas-Wallscheid et al., 2017). (C–F) Bar chart displaying incidence and amplitude for selected genes related to cell cycle and cell cycle–associated DNA repair processes (C), stem cell function (D), LSK flt3⁺ (MPP4) compartment (E), and early lymphoid specification (F). (G) Heatmap of row-normalized ADT UMI counts (log₂, median subtracted) for each cell. Data were from a single pool of LSK cells isolated from four 2-mo-old C57Bl/6J mice.