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. 2023 Nov 1;221(1):e20231035. doi: 10.1084/jem.20231035

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© 2023 Solomon et al.

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Figure 2. — Heterogeneity in CD62L expression and cycling activity of MPP4 populations. (A) Plots of ADT unique molecular identifiers for all captured LSK cells analyzed by CITE-seq. Lower panels show projection on ADT plot of normalized counts for cell cycle genes. (B) Representative FACS plots of phenotypically defined populations of interest in 8-wk-old mouse BM. n = 8 (see Fig. S2 A). (C) qRT-PCR analyses for gene expression in CD62L^+/− MPP4 cells isolated of 8-wk-old control mice. Results are expressed as fold change ± SD relative to CD62L⁺ MPP4 cells, set at 1. n = 3. (D) Mean percentage ± SD of Ki67⁻ cells monitored in Ki67-RFP reporter mice. The right panel displays representative FACS plots showing RFP expression gated on CD62L^+/− MPP4 cells (n = 3). (E) Mean percentages ± SD of Edu incorporating cells. The right panel shows representative FACS plots gated on CD62L^+/− MPP4 cells (n = 4, data from two independent experiments). Student’s t test (C) and two-way ANOVA with Tukey’s post hoc test (D and E): *, P ≤ 0.05, **, P ≤ 0.005; ***, P ≤ 0.0005; ****, P ≤ 0.0001. FMO: Fluorescence minus one.