Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 1;221(1):e20231035. doi: 10.1084/jem.20231035

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Solomon et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S2. — Heterogeneity in cycling activity of CD62L^+/− fractions is unique to the MPP4 population. (A) Average percentage in BM LSK of each population (n = 8). (B) qRT-PCR analyses for gene expression in CD62L^+/− MPP4 cells isolated of 8-wk-old control mice. Results are expressed as fold change ± SD relative to CD62L⁺ MPP4 cells, set at 1. n = 3; Student’s t test; *, P ≤ 0.05; ***, P ≤ 0.0005; ****, P ≤ 0.0001. (C) Representative FACS plots showing RFP fluorescence in hematopoietic populations of 8-wk-old control and Ki67-RFP mice. Fluorescence in wild-type mice was overlaid as fluorescence minus one (FMO). Mean percentage ± SD of RFP⁻ cells indicated on each plot (n = 3). (D) Representative FACS plots and relative percentages of CD62L expression in early BM hematopoietic populations (HSC, MPP5, MPP2, MPP3, MPP4) and lineage-committed progenitors (GMP, CLP) in 8-wk-old mouse (n = 4). (E and F) Percentage ± SD of RFP⁻ cells in total, CD62L^+/− MPP3, and MP (Lin⁻cKit⁺ Sca1⁻) populations (E). Representative FACS plots showing RFP fluorescence in CD62L^+/− MPP3 and MP populations (F) (n = 3). (G) Mean percentages ± SD of Edu incorporation in total, CD62L^+/− MPP3, and MP populations (n = 8). All data are from two independent experiments.