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. 2023 Nov 1;221(1):e20231035. doi: 10.1084/jem.20231035

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© 2023 Solomon et al.

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Figure S3. — Functional properties of slow- and fast-cycling MPP4 subsets. (A) Percentage ±SD of cloning efficiency of HSC and CD62L^+/− MPP4 populations in single-cell methylcellulose-based colony forming assays (n = 4, from two independent experiments). (B) Short-term transplantation (homing) assay: representative FACS plots showing detection of GFP and Tomato donor cells in homing experiment. The graph shows mean percentage ± SD of donor cells in the BM, 36 h after transplantation of CD62L^+/− MPP4 cells (n = 13, from three independent experiments). (C and D) Competitive BM transplantation assays in conditioned recipient using non-limiting (C) and limiting (D) number of Ki67^+/− MPP4 cells. Graphs indicate percentage ± SD of total donor chimerism in PB and percentage ± SD of myeloid and lymphoid cells in donor-derived cells over time. (C) n = 6/group and (D) n = 6–4/group from one experiment. Student’s t test (B) or two-way ANOVA with Sidak’s post hoc test (C and D): *, P ≤ 0.05; ****, P ≤ 0.0001. NS: non-significant; WBM: whole BM; IV: intravenous.