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. 2023 Nov 1;221(1):e20231035. doi: 10.1084/jem.20231035

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© 2023 Solomon et al.

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Figure S4. — Assessment of MPP4 heterogeneity based on CD62L and CD48 expression. (A) Representative FACS plots showing RFP expression in CD48/CD62L-defined MPP4 subsets of Ki67-RFP reporter mice. The graph shows mean percentage ± SD of Ki67-expressing cells in each subset (n = 3). (B) Representative FACS plots showing expression of cMyc, Gfi1, PU.1, and IRF8 monitored in cMyc-GFP, Gfi1-RFP, PU.1-YFP, and IRF8-GFP reporter mice. Data representative of (n) mice. (C and D) Representative FACS plots showing ESAM expression in MPP4 subsets defined by CD48 and CD62L (C) and Ki67-RFP expression in MPP4 subsets defined by ESAM (D) (n = 3). One-way ANOVA with Tukey’s post hoc test (A): **, P ≤ 0.005.