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. 2023 Nov 1;221(1):e20231035. doi: 10.1084/jem.20231035

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© 2023 Solomon et al.

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Figure S5. — Hematopoietic reconstitution in recipient treated with NMA conditioning regimen. (A) Representative FACS plots that show minimal BM hematopoietic alterations in recipient mice; 7-d NMA transplantation (n = 10). (B) Representative FACS plots showing phenotypic evolution over time of the purified LSK populations transplanted in recipient mice conditioned with an NMA regimen (n indicated in Fig. 6 C). (C) Megakaryocytic lineage potential of HSC/MPP populations. Graph indicates percentage ± SD of chimerism in blood CD61⁺ platelets isolated from transplanted recipients. Representative FACS plots showing platelet chimerism in transplanted recipient at day 35 after transplantation (n = 2–3/group). (D) Graph indicates percentage ± SD of donor chimerism in the LSK compartment, 14 d after MPP3 cell transplantation. Representative FACS plots showing phenotypic evolution of the transplanted MPP3 cells (n = 3 from one experiment).