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. 2023 Oct 16;19(16):5275–5289. doi: 10.7150/ijbs.86701

Figure 4.

Figure 4

Omentin-1 enhanced IL-4-dependent polarization of the M2 macrophage. (A) Immunofluorescence staining for CD68, CD86, and CD163 in synovium tissue samples from normal controls and OA patients. Scale bar = 100 µm. (B) TissueFaxs analysis of fluorescence intensity for macrophage polarization in the ACLT model. (C-E) Flow cytometry analysis and qPCR of THP-1 cells incubated with PMA for 24 h prior to stimulation using LPS+IFN-γ or IL-4+IL-13 or Conditioned Medium (CM) from OASF with or without omentin-1 treatment and IL-4 siRNA transfection for 24 h. * p<0.05 vs OASFs group. # p<0.05 vs omentin-1-treated OASFs group.