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. 2023 Oct 2;19(16):5145–5159. doi: 10.7150/ijbs.83906

Figure 1.

Figure 1

PINK1 deficiency aggravates kidney dysfunction, albuminuria, and renal morphological changes in the kidneys of diabetic mice. (A) Western blotting of PINK1 in whole kidney lysate obtained from Pink1+/+ and Pink1-/- mice with normal or high blood glucose levels. (B) % of HbA1c during a 16-week period. (C and D) Levels of serum creatinine (C) and blood urea nitrogen (D) across groups. (E) Quantification of 24-hour albuminuria. (F) Representative photomicrographs of periodic acid-Schiff-stained kidneys of Pink1+/+ and Pink1-/- mice with normal or high serum glucose levels. (G) A semiquantitative assessment of the tubular injury score was performed. (H) Representative photomicrographs of Masson trichrome-stained kidneys of Pink1+/+ and Pink1-/- mice with normal or high serum glucose levels. (I) A semiquantitative assessment of renal fibrosis was performed. Scale bar: (F and H) non-magnified images (upper panels) = 100 μm, magnified images (lower panels) = 50 μm. Values are expressed as mean ± standard error. n = 7 per group, *p < 0.05, **p < 0.01, ***p < 0.001 vs Pink1+/+ NG, #p < 0.05, ##p < 0.01, ###p < 0.001 vs Pink1-/- NG, †p < 0.05, ††p < 0.01 vs Pink1+/+ DM. Abbreviations: PINK1, PTEN-induced serine/threonine kinase 1; HbA1c, glycated hemoglobin; NG, normoglycemia; DM, diabetes mellitus; PAS, periodic acid-Schiff; MT, Masson trichrome.