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. 2023 Oct 2;19(16):5145–5159. doi: 10.7150/ijbs.83906

Figure 2.

Figure 2

PINK1 deficiency increases the expression of profibrotic molecules in the kidneys of diabetic mice. (A and B) mRNA levels (A) and western blotting (B) of α-SMA, fibronectin, E-cadherin, and TGF-β1 in the kidneys of Pink1+/+ and Pink1-/- mice with normal or high serum glucose levels. (C) Representative immunohistochemical staining of α-SMA and fibronectin in the kidneys of Pink1+/+ and Pink1-/- mice with normal or high serum glucose levels. (D and E) A semiquantitative assessment of the staining intensity in the renal tubules was performed. Quantitative analysis of Figure 2B are shown in Figure S1. (C) Scale bar = 100 μm. Data are representative of three independent experiments and is expressed as means ± standard error. n = 7 per group, *p < 0.05, **p < 0.01, ***p < 0.001 vs Pink1+/+ NG, #p < 0.05, ##p < 0.01 vs Pink1-/- NG, †p < 0.05 vs Pink1+/+ DM. Abbreviations: PINK1, PTEN-induced serine/threonine kinase 1; α-SMA, alpha-smooth muscle actin; TGF-β1, transforming growth factor-β1; NG, normoglycemic; DM, diabetes mellitus; IHC, immunohistochemistry.