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. 2023 Oct 2;19(16):5145–5159. doi: 10.7150/ijbs.83906

Figure 5.

Figure 5

PINK1 is involved in mitochondrial electron transport chain complex expressions and cellular oxygen consumption. (A) Western blotting of electron transport chain complex I-V in the kidneys of Pink1+/+ and Pink1-/- mice with normal or high serum glucose levels. (B-D) Representative Seahorse assay traces of HKC-8 cells with control or Pink1 siRNA after treatment with 5- or 30-mM D-glucose and the quantification of OCR at basal and maximal respiration. (E-G) Representative Seahorse assay traces of control or GFP-Pink1 infected HKC-8 cells after treatment with 5- or 30-mM D-glucose and the quantification of OCR at basal and maximal respiration. (A) n = 6 per group, (B-G) Data are representative of five independent experiments and are expressed as means ± standard error. **p < 0.01, ***p < 0.001 vs Pink1+/+ NG, #p < 0.05, ##p < 0.01, ###p < 0.001 vs Pink1-/-, NG, †p < 0.05, ††p < 0.01, †††p < 0.001 vs Pink1+/+ DM. Abbreviations: PINK1, PTEN-induced serine/threonine kinase 1; NG, normal glucose; DM, diabetes mellitus; OCR, oxygen consumption rate; OE, overexpression.