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. 2023 Oct 2;19(16):5145–5159. doi: 10.7150/ijbs.83906

Figure 6.

Figure 6

PINK1 regulates the generation of reactive oxygen species in renal tubular epithelial cells under high glucose conditions. (A and B) Representative immunohistochemical staining of 8-OHdG in the kidneys of Pink1+/+ and Pink1-/- mice with normal or high serum glucose levels. A semiquantitative assessment of the staining intensity in the renal tubules was performed. (C) Representative images of MitoSOX red staining of superoxide radicals and their quantification in HKC-8 cells with control siRNA or Pink1 siRNA after treatment with 5- or 30-mM D-glucose. (D) Immunofluorescence images showing DHE staining and their quantification in HKC-8 cells with control or Pink1 siRNA after treatment with 5- or 30-mM D-glucose. (E) Representative images of MitoSOX red staining of superoxide radicals and their quantification in control or GFP-Pink1 infected HKC-8 cells after treatment with 5- or 30-mM D-glucose. (F) Immunofluorescence images of DHE staining and their quantification in control or GFP-Pink1 infected HKC-8 cells after treatment with 5- or 30-mM D-glucose. Scale bar: (A) 100 μm, (C-F) 100 μm. (A) n = 7 per group, (C-F) Data is representative of three independent experiments and expressed as means ± standard error. n = 3 per group. **p < 0.01, ***p < 0.001 vs Pink1+/+ NG, #p < 0.05, ##p < 0.01, ###p < 0.001 vs Pink1-/-, NG, †p < 0.05, ††p < 0.01, †††p < 0.001 vs Pink1+/+ DM. Abbreviations: PINK1, PTEN-induced serine/threonine kinase 1; NG, normal glucose; DM, diabetes mellitus; 8-OHdG, 8-hydroxy-2′-deoxyguanosine; DHE, dihydroethidium; OE, overexpression.