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. 2023 Oct 2;19(16):5145–5159. doi: 10.7150/ijbs.83906

Figure 7.

Figure 7

PINK1 inhibits necroptosis in renal tubular epithelial cells under high glucose conditions. (A) Representative immunohistochemical images of TNFα and p-MLKL in the kidneys of Pink1+/+ and Pink1-/- with normal or high blood glucose levels. Scale bar = 50 μm. (B) Western blotting of necroptosis-related proteins in primary renal tubular epithelial cells of Pink1+/+ and Pink1-/- mice after treatment with 5- or 30-mM D-glucose. (C) Western blotting of necroptosis-related and profibrotic proteins in necrostatin-1-treated HKC-8 cells under 5- or 30-mM D-glucose. (D) Quantification of the mRNA expression of necroptosis-related molecules in HKC-8 cells with control or Pink1 siRNA after treatment with 5- or 30-mM D-glucose. (E) Western blotting of necroptosis-related proteins in control or PINK1 siRNA-treated HKC-8 cells under 5- or 30-mM D-glucose. (F) Quantification of the mRNA expression of necroptosis-related molecules in control or GFP-Pink1 infected HKC-8 cells after treatment with 5- or 30-mM D-glucose. (G) Western blotting of necroptosis-related proteins in PINK1-overexpressed HKC-8 cells after treatment with 5- or 30-mM D-glucose. Quantitative analysis of Figure 7B, 7C, 7E, and 7G are shown in Figure S3. (B-G) Data are representative of three independent experiments and are expressed as means ± standard error. *p < 0.05, **p < 0.01, ***p < 0.001 vs Pink1+/+ NG, ###p < 0.001 vs Pink1-/- NG, ††p < 0.01, †††p < 0.001 vs Pink1+/+ DM. Abbreviations: PINK1, PTEN-induced serine/threonine kinase 1; NG, normal glucose; DM, diabetes mellitus; TNFα, tumor necrosis factor α; MLKL, mixed lineage kinase domain-like pseudokinase; RIPK, receptor-interacting protein kinase; α-SMA, alpha-smooth muscle actin; OE, overexpression.