Skip to main content
. 2023 Dec 8;8:366. Originally published 2023 Aug 25. [Version 2] doi: 10.12688/wellcomeopenres.19666.2

Figure 10. Correlative XRH and conventional histology imaging of a human head and neck tumour specimen.

Figure 10.

Specimen was scanned on a cassette at 15 μm voxel (3D pixel) size without staining. Serial sections were taken after μCT and stained with H&E. Top row: Side-by-side visualisation of matching XRH and conventional histology images on the left ( a, b); Augmented tomography in the middle ( c) is made possible by fusing conventional histology images with XRH data to enhance the specific information presented by the mainly structural μCT data; Volumetric visualisation of the whole tissue block on the right ( d) offers 3D context and overview of the microanatomy. Bottom row: Close-up images on the central part of the volume shown in ( a) & ( b). Single XRH slice on the left ( e), H&E slice in the middle ( f), and an MIP image ( g) uncovering the volumetric development of micro-vasculature on the right.