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. 2023 Oct 19;14:1249436. doi: 10.3389/fimmu.2023.1249436

Figure 2.

Figure 2

INX-14 acts in the germline of C elegans to suppress resistance to PA14. (A–D) Representative images (A, C) and fluorescence intensity (B, D) of GFP-expressing PA14 bacteria accumulated in the intestine (A, B) or pharynx (C, D) of wild-type and inx-14(ag17) mutants after exposed to P. aeruginosa for 24 (h) (E) Quantification of the colony-forming units (CFU) of wild-type and inx-14(ag17) worms exposed to PA14. Data are presented as mean ± SEM. (F) Survival of wild-type worms fed on vector control or inx-14 RNAi bacteria prior to PA14 exposure. (G–K) PA14 survival assays following tissue-specific RNAi in the germline (DCL569) (G), intestine (VP303) (H), neurons (TU3311) (I), muscle (WM118) (J), hypodermis (NR222) (K) of animals fed on vector control or inx-14 RNAi bacteria prior to PA14 exposure. (L, M) Survival assay of wild-type, inx-14(ag17), and transgenic lines expressing INX-14 in the germline (L) and in the intestine (M). All experiments were repeated at least three times. The number of animals analyzed is indicated in (B) and (D). Scale bar, 10 μm. Statistical significance was determined by log-rank test for survival assays, or unpaired Student’s t-test for (B), (D), or non-parametric test for (E). ***P < 0.001; n.s., not significant. The exact P values of statistics for all survival assays are listed in Supplementary Table S1 .