FIG. 1.

Restriction map of the cosmid clone 13-6-4 (A) and a more detailed map of the region that contains the tutD and tutE genes (B). Four open reading frames contained in the SacII/EcoRI region are indicated by arrows. Subclones tested for complementation are included. The abilities of cosmid clone 13-6-4 and the subclones to complement the tutD17 and tutE21 mutations are also indicated: +, full complementation; −, no complementation. Abbreviations: B, BamHI; C, ClaI; H, HindIII; N, NcoI; P, PstI; R, EcoRI; Sa, SacII; Sc, SacI. Not all sites are shown in panel A. Sites blocked by methylation are omitted. X, the site is lost in the construct.