TABLE 1.

Complementation of the tutD17 mutation by the site-directed mutations constructed in the tutD gene.

Plasmid	% Toluene utilized	% Monounsaturated, benzylsuccinic acid-derived, compound producedc
pPWC4-CLSaca	100	100
pRK415b	23.5 ± 6.4	1.3 ± 0.1
pPWC4-CLSac-G828A	34.2 ± 9.7	13.0 ± 3.8
pPWC4-CLSac-C492A	17.7 ± 5.4	1.8 ± 0.1

^aThe plasmid carrying the unaltered clone, serving as a positive control. 

^bThe vector alone, serving as a negative control. 

^cNormalized to 100% for pPWC4-C_LSac, the positive control.