TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli strains | ||
| C2110nal | Nalr derivative of C2110, polA | 10 |
| DH5 | Nalr, recA | Bethesda Research Laboratories |
| HB101 | Smr, recA | 4 |
| R. leguminosarum bv. viceae 128C1 | TFXs strain used in trifolitoxin assays | LiphaTech, Inc. |
| Sinorhizobium sp. strain ANU280 | Smr derivative of Sinorhizobium sp. strain NGR234 | 7 |
| Plasmids | ||
| pTR102 | par, Tcr | 47 |
| pTFX24 | pBluescript II KS+::7.1-kb MluI fragment of pTFX1 | 5 |
| pHoKmGus | Tn3HoHo derivative, Tn3 (Kmr AprtnpA, promoterless uidA) | 2 |
| pSShe | pACYC184::tnpA, Cmr | 38 |
| pRK2013 | ColE1 mobilization helper (Tra+ Mob+), Kmr | 13 |
| pRK2073 | ColE1 mobilization helper (Tra+ Mob+), Spr Smr | 10 |
| pLAFR1 | RK2-derived broad-host-range cosmid vector, Tcr | 14 |
| pHU52 | pLAFR1 with 30-kb insert containing the hup locus, Tcr | 19 |
| pTn3PAR | 3.2-kb par locus cloned into the ClaI site of pHoKmGus | This study |
| pTn3TFXPAR | 10.4-kb fragment containing tfx operon and par locus cloned into the ClaI site of pHoKmGus | This study |
| pLAFR1::Tn3 | pLAFR1 carrying Tn3Gus within tetA, Tcs Kmr | This study |
| pLAFR1::PAR | pLAFR1 carrying Tn3PAR within tetA, Tcs Kmr | This study |
| pTFXPAR | pLAFR1 carrying Tn3TFXPAR within tetA, Tcs Kmr | This study |
| pHUTn3 | pHU52 carrying Tn3Gus within tetA, Tcs Kmr | This study |
| pHUPAR | pHU52 carrying Tn3PAR within tetA, Tcs Kmr | This study |
| pHUTFXPAR | pHU52 carrying Tn3TFXPAR within tetA, Tcs Kmr | This study |