TABLE 2.
Retention of cell tracking dyes by S. cerevisiae N1
| Time (h) | BR orange fluorescencea | CT-Green BODIPY green fluorescence | CT-Green green fluorescence |
|---|---|---|---|
| 0 | 590 (59) | 600 (83) | 500 (154) |
| 4 | 490 (48) | 640 (78) | 300 (88) |
| 25 | 430 (56) | 690 (132) | 180 (63) |
a
CT-Green BODIPY and CT-Green were measured in the green fluorescence channel (FL1) (525 ± 5 nm), and BR was measured in the orange fluorescence channel (FL2) (575 ± 5 nm). All values are expressed in arbitrary fluorescence units and are averages (standard deviations) of data from three experiments. Unstained cells had typical mean autofluorescence values of 8 to 12 in both FL1 and FL2.