Figure 2. Hypoxia suppresses the release of inflammatory cytokines and retinoic acid-inducible gene (RIG)-I like receptors (RLRs) by renal proximal tubular epithelial cells (RPTECs) in response to polyIC treatment. RPTECs were cultured under normoxic or hypoxic (2% O2) conditions for 2 h and stimulated with polyIC for an additional 6 and 24 h, respectively. Quantitative reverse transcription-polymerase chain reaction (A-E) and western blotting analysis (E, F) showed that hypoxia markedly reduced RLRs mRNA and protein expression levels and inflammatory mediator mRNA expression levels. Data are presented as mean±SEM (n=3/group). Bars indicate significant differences of **p<0.01 and ***p<0.001.
