Figure 6. HIF1α knockdown increased RIG-I (A), MDA5 (B), and IFNβ (C) mRNA expression levels in renal proximal tubular epithelial cells (RPTECs) after polyIC treatment compared to those under normoxic conditions, with no significant difference in IL6 (D) and TNFα (F) mRNA expression levels. RIG-I (F) and MDA5 (G) protein expression levels were measured using western blotting analysis, and their results were comparable to that of qRT-PCR. LDH in the supernatant was presented as a percentage of cytotoxicity (H). HIF1α knockdown increased LDH activity in the supernatant of RPTECs under hypoxic conditions or after polyIC treatment. Data are presented as mean±SEM (n=3/group). Bars indicate significant differences at *p<0.05, **p<0.01, and ***p<0.001.