FIG. 6.

Effect of chaperone coexpression on disaggregating Cryj2 in the ΔrpoH mutant at 20°C. A pair of NK161 (ΔrpoH) strains harboring pKCJ2l and pKJE7 or pGro7 were grown at 20°C, each chaperone team was induced with arabinose (Ara), and Cryj2 was induced with IPTG for 3 h. The levels of expression of chaperones (A) and the levels of expression of Cryj2 before (B) and after (C) fractionation were determined essentially as described in the legends to Fig. 2 and 4. Lanes S, soluble fractions; lanes I, insoluble fractions.